We seek an alternative high-throughput strategy that will obviate the need for EEG/EMG recording. Thus, the necessity for surgery, time to recover from surgery, and scoring of large numbers of epochs adds expense and makes studies of sleep in mice very labor intensive. Moreover, scoring of EEG/EMG records is labor intensive: if states are assessed in 10-second epochs across a 24-hour period, there are 8,640 epochs to be scored, whereas with 4-second epochs, there are 21,600 epochs. This technique requires surgery on mice with the implantation of electrodes mice cannot be studied until they recover from the surgery. 3 Use of these new mouse resources requires evaluation of the effect of knockout of a specific gene on sleep and its substages as well as wakefulness.Ĭurrently, this evaluation is performed by assessing changes in the electroencephalogram (EEG) and electromyogram (EMG). There are currently large numbers of mice available with knockout of specific genes.

Transgenic strategies typically create altered gene function on a C57BL/6J background by using selective breeding strategies to minimize the effects of genetic background.

These include the analysis of changes in the transcriptome with sleep, wake, and sleep deprivation (for review, see 2) as well as the use of specific transgenic mice. Multiple strategies are used to identify relevant genes (for review, see 1). A major focus of current research in mice is to elucidate gene products that (1) regulate sleep and wake, (2) are regulated by sleep and wake, or (3) are affected by sleep deprivation.

Elucidate 5am track list